Analysis of Genetic Variation among Accessions of Wild and Cultivated Species of Amaranthus Based on Rapd and Sds-page Markers

نویسندگان

  • REEMA SRIVASTAVA
  • BIJOY K. ROY
چکیده

Genetic diversity and relationships among 12 cultivated and wild Amaranthus species were examined using protein and RAPD markers. High level of genetic diversity was common within species contrary to genetic uniformity within most accessions. On average, the polymorphism reached 42.60% among the cultivars but raised to 46.88% in the wild counterpart. The seed protein content varied in the 11.80-17% range and seeds of amaranth proved highly nutritive. The SDS-PAGE analysis indicated that such proteins in case of amaranths were highly heterogenous with 8-18 bands. Based on the electrophoretic mobility, such bands were assigned four zones (A, B, C and D). The RAPD analyses of Amaranthus species are further substantiated by the seed protein profile, indicating that a combination of such approaches can offer relatively reliable parameters to unravel genetic diversity.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Determination of genetic uniformity in transgenic cotton plants using DNA markers (RAPD and ISSR) and SDS-PAGE

One concern about using transgenic plants is the genetic variation that occurred from theirs tissue culture and regeneration. Molecular markers are an important element for efficient and effective determination of genetic variation. The present work was carried out to assess the genetic uniformity of transgenic cottons (Bt and chitinase lines), using RAPD, ISSR molecular markers and SDS-PAGE an...

متن کامل

Genetic variation of some Iranian Hyoscyamus Landraces based on seed storage protein

The genus Hyoscyamus belongs to the tribe Hyoscyameae Miers of Solanaceae family. Variation in protein bands elaborates the relationship among the collections from various geographical regions. In this study the seed storage protein diversity of 19 accessions of Hyoscyamus (H. niger, H. reticulatus and  H. pusillus) from West Azerbaijan (Iran) was investigate...

متن کامل

Assessment of Genetic Structure and Variation of Native Berberis Populations of Khorasan Provinces (Iran) Using AFLP Markers Versus Morphological Markers

Seedless barberry (Berberis vulgaris L. var. asperma) is one of the few crops that is only cultivated in eastern parts of Iran. As a new crop there has not been any study to identify phylogenetic relationships of this plant with other related species existing in Iran. In this study, Amplification fragment length polymorphism (AFLP) markers based on four selected primer combinations (EcoRI/Tru1I...

متن کامل

Genetic Relationships among Pistacia Species Studied By Morphological Characteristics and RAPD Marker

The aim of this research was to study 33 Pistachio accessions and determine their genetic relationships. Thirty-one morphological characters (17 quantitative and 14 qualitative) together with Randomly Amplified Polymorphic DNA (RAPD) marker data were used for this purpose. Factor analysis was used to determine the effective characteristics and the number of main factors which determined seven m...

متن کامل

Genetic and Morphological Diversity of Wild Mint "Mentha longifolia (L.) Hudson subsp. noeana (Briq.) Briq." in South and Southeastern Iran

The wild mint "Mentha longifolia (L.) Hudson subsp. noeana (Briq.) Briq." is an important medicinal plant which contains several worthy components like: menthone, pulegone, pipertenon, menthol, thymol, and carvone in its essential oil. This plant grows extensively in center of Asia including Iran. Because of so worthy components and its big role in traditional and modern medicine, the wild mint...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2012